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(), . . , . ( ), [Reid G.,2001].ʠ Lactobacillus Bifidobacterium, Streptococcus, Lactococcus, Enterococcus, Bacillus Saccharomyces [ . ., 1998; HolzapfelW.H.etal.,2001].





[..,1998;...,2004;AlvarezOlmosM.I.et al., 2001; Agarwal K. N. et al., 2002]. : , ,, , [DeVreseM.etal.,2001;IsolauriE.etal.,2001;PereiraD.I.etal.,2002; GillH.S.etal.,2004].   , ,[KaizuH. et al.,1993; Wang Y. C. et al.,2006; Virtanen T. et al.,2007; Mikelsaar M. et al., 2009].  () ,, [Kaizu H. et al.,1993; Lin M. Y. et al., 1999; Stecchini M. L. et al.,2001; Saide J. A. O. et al.,2005; Aoi W. et al.,2007; MikelsaarM.etal.,2009]. , . , , 軠, [..,1998;PedrosaM.C.etal.,1995;HondaH.etal.,2007]. , ,,蠭 (), , [Marteau P. et al., 1990; De Vrese M. et al., 2001; LeBlanc J. G. et al., 2005]. (, ) [ . ., 1998; Nemeth K. et al., 2003; Crozier A. et al., 2009].

͠堹093 ໠(20082010.). .





:

1. invitro;

2. Lactobacilluscasei114001 ;

3. L.casei114001 ; 4. ; 5. L. casei 114001 ; 6. L. casei 114001 (3έL). ., L. casei L. fermentum ME3 ()ˠ.頭蠭 L. fermentum ME3 L.casei.,L.casei114001 , ˠ() , . L. casei 114001 , . , L. casei 114001 .L.casei 114001 . , . . L. casei 114001, , . 252010 Ƞ.I,ՠ I⠫廠 (, 2007, 2008, 2009), 3 (, 2008), 2009 (, 2009), Ƞ (, 2009), V 軠(,2009).

11 , 3 , .

178, , , , , , , 35 40 . 43 280.

۠Ƞ۠

 Lactobacillusdelbrueckiisubsp.bulgaricus, Streptococcus thermophilus Lactobacillus casei, () Lactobacillus fermentum ME3, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSM 14241). 20% 5,3%Π蠭70. , . : , , (109 /), .蠭70.

vitro HbH2O2 [ . . ., 1997, 1998] ͭFe2+ ˠ, [ . . ., 1972]. Fe3+ FRAP (ferric reducing antioxidant power) [Benzie F. F. I. et al., 1990]. [NishikimiM.etal.,1971].

API ZYM (BioMerieux, ).   [Kim K.Y. et al.,2008], [Djouzi Z.etal.,1997], [Yoon M. Y. et al., 2008], [Martini M. C. et al., 1991], [DjouziZ.etal.,1997]. in vivo in vivo , . Lactobacillus casei 114001 , , 14(/)0,5 (2109 /), .

L. casei 114001 , , 1 21 / 0,5 (108 /), 2 0,5 ࠫ(Π), L.casei114001(108/). . L. casei 114001 .   81/0,5 , 2 L. casei 114001 2,81010 /. 24 (/) CCl4 (1:1)0,51, .5 ..24 /CCl40,05, 0,10,0,250,50/2,5,5,12,525% . L. casei 114001 0,4%.   141/0,5 , 2 L. casei 114001 2109/.

12. , , , .

 [Uchiyama M. et al.,1978]. , [.. .,1997,1998][BenzieF.F.I.etal.,1990],.Š TAS (Total Antioxidant Status) RANDOX (Randox Laboratories, ).   [Aebi H. E.,1984],[Nishikimi M. et al.,1971],[Flohe L. et al.,1984], [Habig W. H. et al., 1974], [Cai Q. et al., 1996], [Benson A. et al., 1980], ԭ [Burchell B. et al.,1981],[Lin W. et al.,2005].  [ ., 1980]1. , [Djouzi Z. et al.,1997].  [ManachC.et al.,1995].[LowryO.H.etal.,1951].

ANOVA t Statistica.

۠ՠɠ

, invitro(.1). . ˠ . L. casei L. fermentum Π4365,8%ˠ57,9 89,5%, L. bulgaricus S. thermophilus 15,9 17,9%, . .1.(), c L.bulgaricus L.bulgaricus ...,........,.....

L.bulgaricus L.bulgaricus L.casei L.casei L.casei L.fermentumŭ S.thermophilus S.thermophilus S.thermophilus S.thermophilus S.thermophilus S.thermophilus /14L.casei114001 2109 / . (.1) Š TAS 7%30%, . (.2) (92%) ( 56%), ( 21%)(72%).Ӡ,L.casei,Š (.3).

, L. casei 114001 invivo, , , .

, , p0, .1.(FRAP), (TAS),()

FRAP -

. 2. (FRAP), Š ,L.casei .3.(FRAP)Š . ,, /21L.casei114001 108/(1) L. casei 114001 (2 ), (.2). 1 2 28 32%, , 26 18%, . 12 Š2915%()3430%, .Š 123130%,.

.2.,L.casei(1 )(2 ̠Fe2+ (FRAP),̠Fe2+ ̠Fe2+ Mm.

, L. casei 114001 .

(/) CCl4 0,05, 0,10,0,250,50/ (475% CCl4),(180% CCl4) ( 177% CCl4).

CCl4 , , , ԭ (. 4). %

FRAP -

*堭0,05 .4.(FRAP) 24/CCl4 CCl4 ( ) ( ) CCl4 (. 5), . .5. 24/CCl40,050,50/..

, , CCl4 , . 0,05CCl4/..

Lactobacillus CCl4 (1 ) ,30%, 8 8 , Š. 5640%,(.6). 23%, 15%,22%,ԭ35%.  , CCl4, 39%. CCl4 , L. casei 114001 (2 ) , 38.Š , CCl4 , , . .

FRAP - GSH

. 6. (FRAP), 24 CCl4(1)CCl4+L.casei(2) CCl4 L. casei 114001, , , CCl4 (.7), CCl4.

.7. 24CCl4(1)CCl4+L.casei(2 , L. casei 114001 CCl4 . API ZYM (19 ,), .  ,L. casei L. fermentum L.bulgaricusS. thermophilus(.3).L.fermentumME3 , . L. casei L. fermentumME3頭蠭, , . , ,L.bulgaricusS.thermophilus. .3. L.fermentumME L.bulgaricus L.bulgaricus L.bulgaricus S.thermophilus S.thermophilus /14L.casei114001 2109/蠭, (.8).  ,L.casei114001, , 52% (. 9). ,帠 [Gorbach S.L. et al.,1990; RowlandI.R.etal.,1998;KarthikkumarV.etal.,2009]. % .8. .9.蠭 ԭ ,L.casei ,L.casei L.casei114001, , , .

0,4% (10%)Š(27%) ( 19%) (. 10). (36%)Š(68%)(.11). (.12). .10.(FRAP), (TAS),Š(1 )+L.casei(2)

FRAP -

.11.(FRAP),Š ,(1) +L.casei(2)

FRAP , ,

.12.(FRAP),Š (1)+L.casei(2 L. casei , , . 45% 21% , L. casei 114001. 61%,Š94%. , , .   ,1, (18%) Š 蠖 42% ,Π13%.

, L. casei 114001, ԭ 176 200% , (.13). , , , . (.14),0,4% 42% .L.casei蠭 2, 17%.

% )+L.casei(2 (37%) , L.casei.,, .

1. , (Lactobacillus delbrueckii subsp. bulgaricus, L. casei, L. fermentum, Streptococcus salivarius subsp. thermophilus) , L. casei L. fermentum . 2. L.casei114001 ( 21%) ( 72%) , , (30%).

3. L. casei 114001 , CCl4. (, ԭ ), , CCl4 . 4. L. bulgaricus, L. casei, L. fermentum, S. thermophilus. L.caseiL.fermentum. 5. L. casei 114001 ԭ ( 29%) 锠 (45%). 6. L. casei 114001 , , .

ʠ,ՠΠŠ

, 1. .., .. 頖.//. 2009.2..1823. 2. .., .., .., .., .., .., .. Lactobacillus casei 114001 , .//..2009.5..2430.

3. ..,..,..,.., .., .., .., .., .. .//.ꠖ2009.1..1218.

4. .., .., .. ? // IX ⠫.,2007..86.

5. .. in vitro. // X ⠫.,2008. .111112.

6...,..,..,..,.., .., .. , . // 3 .,2008..154.

7. .. , . // 2009.. ,2009..1920.

8...,.. ,.// . ,2009..7374.

9. .., .., .. Lactobacilluscasei114001 . // XI ⠫.,2009..78.

10. ..,.., .. Lactobacilluscasei114001.//XI ⠫. ,2009..169170.

11. ..,..,..,.., ..,..,.. , . // V . ,2009..122124.

ʠ

Š ݠ 렖 렖 ꠖ ꠖ 𠖠 Ϡ Р Ҡ Π Ҡ Š Π ˠ Ġ Π ԭҠ ˠ Р FRAPferricreducingantioxidantpower GSH TAStotalantioxidantstatus

 
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